Journal: CNS Neuroscience & Therapeutics

Article Title: ErbB2 pY ‐1248 as a predictive biomarker for Parkinson's disease based on research with RPPA technology and in vivo verification

doi: 10.1111/cns.14407

Figure Lengend Snippet: Western blot analysis of ErbB2 pY‐1248 , ErbB2, and ErbB1 in cellular PD model. (A, C) Representative images of western blot of ErbB2 pY‐1248 , ErbB2, and ErbB1 in extracted protein lysates from SH‐SY5Y cells treated with 6‐OHDA for 6 h. (B, D) Grayscale value analysis of the immunoblots. The band intensity of ErbB2 pY‐1248 and ErbB1 were normalized to ErbB2 and β‐Actin, respectively. It is presented as mean ± SEM. *** p < 0.001 vs. Ctl.

Article Snippet: After blocking with 5% skim milk in TBST for 2 h at room temperature, membranes were incubated overnight at 4°C with primary antibodies against β actin (1:5000, A5441, Sigma), ErbB2 pY‐1248 (1:1000, AF1768, R&D Systems), ErbB2 (1:400, sc‐377,344, Santa Cruz Biotechnology), and ErbB1 (1:400, 2232S, Cell Signaling Technology).

Techniques: Western Blot

Journal: CNS Neuroscience & Therapeutics

Article Title: ErbB2 pY ‐1248 as a predictive biomarker for Parkinson's disease based on research with RPPA technology and in vivo verification

doi: 10.1111/cns.14407

Figure Lengend Snippet: Western blot analysis of ErbB2 pY‐1248 in mouse PD model. (A) Representative images of western blot of ErbB2 pY‐1248 in the mouse brain tissues. (B) Grayscale value analysis of the immunoblots. The band intensity of ErbB2 pY‐1248 was normalized to β‐Actin. *** p < 0.001 vs. Ctl.

Article Snippet: After blocking with 5% skim milk in TBST for 2 h at room temperature, membranes were incubated overnight at 4°C with primary antibodies against β actin (1:5000, A5441, Sigma), ErbB2 pY‐1248 (1:1000, AF1768, R&D Systems), ErbB2 (1:400, sc‐377,344, Santa Cruz Biotechnology), and ErbB1 (1:400, 2232S, Cell Signaling Technology).

Techniques: Western Blot